Test No. HR Steroidogenesis Assay - Books - OECD iLibrary

Toxicology

in vitro steroidogenesis assay

The reference chemicals may be used to validate in vitro steroidogenesis models. Joint Accredited with multiple accreditations, including:. Subscribe to the feed. Drug toxicity, often manifested as liver toxicity and cardiotoxicity, is a key reason for drug attrition. Candidate reference chemicals were identified from a review of HR and gonad-derived in vitro assays used in methods validation and published in the scientific literature. By continuing to browse the site you are agreeing to our use of cookies. This website uses cookies to deliver its services as described in our Cookie Policy.

Highlights

A total of 29 chemicals affecting androgen and estrogen levels satisfied all criteria for positive reference chemicals, while an additional set of 21 and 15 chemicals partially fulfilled criteria for positive reference chemicals for androgens and estrogens, respectively. The human HR adreno-carcinoma cell line, used for the assay, expresses genes that encode for all the key enzymes for steroidogenesis. In terms of sensitivity, steroid production in the HR assay was most sensitive for detecting the effects of fadrozole, fenarimol, and prochloraz, but was less sensitive than the fathead minnow ovary explant assay to the effects of ketoconazole and vinclozolin. The identified chemicals included pesticides, pharmaceuticals, industrial and naturally-occurring chemicals with the capability to increase or decrease the levels of the sex hormones in vitro. Better navigate the business aspects of medicine and stay on top of the changing healthcare landscape. Higley b John L.

However, the ovary explant assay was more predictive of in vivo effects of the six chemicals on fathead minnows than the HR system. Further characterization of autoregulatory capacities, interaction of steroid-hormone receptor pathways with steroidogenesis, and metabolic capabilities of each system are needed for either system to provide clear and informative insights regarding a chemical's mechanism of action. Overall, however, results of this study suggest that both the HR and fathead minnow ovary explant assays have utility for identifying endocrine-active chemicals in screening-type applications.

Author links open overlay panel Daniel L. Villeneuve a Gerald T. Ankley a Elizabeth A. Makynen a Lindsey S. Blake a Katie J. Greene a Eric B. Higley b John L. Essential reference tools, including a drug-interaction checker, medical calculators, and a pill identifier. Joint Accredited with multiple accreditations, including:. Get helpful advice on your cases from a community of physicians. Gain Essential Business Knowledge. Better navigate the business aspects of medicine and stay on top of the changing healthcare landscape.

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Iamges: in vitro steroidogenesis assay

in vitro steroidogenesis assay

Toxicology in Vitro Volume 47 , March , Pages The OECD Guidelines for the Testing of Chemicals is a collection of about of the most relevant internationally agreed testing methods used by government, industry and independent laboratories to identify and characterise potential hazards of chemicals. Name required Email address required Job title required Company required Contact phone number How did you hear about us?

in vitro steroidogenesis assay

This group of tests covers health effects.

in vitro steroidogenesis assay

In vitro steroidogenesis assay course certificates and optional CME. The identified chemicals included pesticides, pharmaceuticals, industrial and naturally-occurring chemicals with the capability to increase or decrease the levels of the sex hormones in vitro. Find out more here. Data are expressed as fold change relative to the solvent control and the Lowest-Observed-Effect-Concentration. By using this website, you agree to the use of cookies.